About Tiago

Our research interests are focused on the understanding of the molecular mechanisms which lead to neurodegeneration in diseases such as Parkinson’s, Huntington’s, or Alzheimer’s disease. These diseases are intimately associated with protein misfolding and aggregation in specific regions of the brain.
Because the molecular pathways involved in protein homeostasis are highly conserved, we employ a wide variety of model organisms, from the simple but powerful budding yeast to mammalian cell culture and mice, to study the origin of the problems.
Our ultimate goals are to develop novel therapeutic approaches for these and other related disorders. We are working closely together with clinicians in order to accelerate drug discovery efforts, translating basic research into clinical applications that will improve the lives of patients.

List of Heat-Net collaborative publications with at least 1 member of the consortium (from other Heat-Net group) from the last 5 years.

  • Glycation potentiates α-synuclein-associated neurodegeneration in synucleinopathies.
    Vicente Miranda H, Szego ÉM, Oliveira LMA, Breda C, Darendelioglu E, de Oliveira RM, Ferreira DG, Gomes MA, Rott R, Oliveira M, Munari F, Enguita FJ, Simões T, Rodrigues EF, Heinrich M, Martins IC, Zamolo I, Riess O, Cordeiro C, Ponces-Freire A, Lashuel HA, Santos NC, Lopes LV, Xiang W, Jovin TM, Penque D, Engelender S, Zweckstetter M, Klucken J, Giorgini F, Quintas A, Outeiro TF.
    Brain : a journal of neurology. 2017 May 1;140(5):1399-1419. doi: 10.1093/brain/awx056.
    PubMed

  • Alpha-synuclein prevents the formation of spherical mitochondria and apoptosis under oxidative stress.
    Menges S, Minakaki G, Schaefer PM, Meixner H, Prots I, Schlötzer-Schrehardt U, Friedland K, Winner B, Outeiro TF, Winklhofer KF, von Arnim CA, Xiang W, Winkler J, Klucken J.
    Scientific reports. 2017 Feb 22;7:42942. doi: 10.1038/srep42942.
    PubMed

  • Systematic comparison of the effects of alpha-synuclein mutations on its oligomerization and aggregation.
    Lázaro DF, Rodrigues EF, Langohr R, Shahpasandzadeh H, Ribeiro T, Guerreiro P, Gerhardt E, Kröhnert K, Klucken J, Pereira MD, Popova B, Kruse N, Mollenhauer B, Rizzoli SO, Braus GH, Danzer KM, Outeiro TF.
    PLoS genetics. 2014 Nov 13;10(11):e1004741. doi: 10.1371/journal.pgen.1004741. eCollection 2014 Nov.
    PubMed

  • The small GTPase Rab11 co-localizes with α-synuclein in intracellular inclusions and modulates its aggregation, secretion and toxicity.
    Chutna O, Gonçalves S, Villar-Piqué A, Guerreiro P, Marijanovic Z, Mendes T, Ramalho J, Emmanouilidou E, Ventura S, Klucken J, Barral DC, Giorgini F, Vekrellis K, Outeiro TF.
    Human molecular genetics. 2014 Dec 20;23(25):6732-45. doi: 10.1093/hmg/ddu391. Epub 2014 Aug 4.
    PubMed

  • Heat-mediated enrichment of α-synuclein from cells and tissue for assessing post-translational modifications.
    Vicente Miranda H, Xiang W, de Oliveira RM, Simões T, Pimentel J, Klucken J, Penque D, Outeiro TF.
    Journal of neurochemistry. 2013 Sep;126(5):673-84. doi: 10.1111/jnc.12251. Epub 2013 Apr 25.
    PubMed

  • Tau enhances α-synuclein aggregation and toxicity in cellular models of synucleinopathy.
    Badiola N, de Oliveira RM, Herrera F, Guardia-Laguarta C, Gonçalves SA, Pera M, Suárez-Calvet M, Clarimon J, Outeiro TF, Lleó A.
    PLoS One. 2011;6(10):e26609. doi: 10.1371/journal.pone.0026609. Epub 2011 Oct 24.
    PubMed

  • Secretion and Uptake of α-Synuclein Via Extracellular Vesicles in Cultured Cells.
    Gustafsson G, Lööv C, Persson E, Lázaro DF, Takeda S, Bergström J, Erlandsson A, Sehlin D, Balaj L, György B, Hallbeck M, Outeiro TF, Breakefield XO, Hyman BT, Ingelsson M.
    Cell Mol Neurobiol. 2018 Nov;38(8):1539-1550. doi: 10.1007/s10571-018-0622-5. Epub 2018 Oct 4.
    PubMed

  • Cellular Uptake of α-Synuclein Oligomer-Selective Antibodies is Enhanced by the Extracellular Presence of α-Synuclein and Mediated via Fcγ Receptors.
    Gustafsson G, Eriksson F, Möller C, da Fonseca TL, Outeiro TF, Lannfelt L, Bergström J, Ingelsson M.
    Cell Mol Neurobiol. 2017 Jan;37(1):121-131. doi: 10.1007/s10571-016-0352-5. Epub 2016 Mar 10.
    PubMed 

  • Off-pathway α-synuclein oligomers seem to alter α-synuclein turnover in a cell model but lack seeding capability in vivo.
    Fagerqvist T, Näsström T, Ihse E, Lindström V, Sahlin C, Tucker SM, Kasaryan A, Karlsson M, Nikolajeff F, Schell H, Outeiro TF, Kahle PJ, Lannfelt L, Ingelsson M, Bergström J.
    Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis. 2013 Dec;20(4):233-44. doi: 10.3109/13506129.2013.835726. Epub 2013 Sep 20.
    PubMed

  • Extracellular alpha-synuclein oligomers modulate synaptic transmission and impair LTP via NMDA-receptor activation.
    Diógenes MJ, Dias RB, Rombo DM, Vicente Miranda H, Maiolino F, Guerreiro P, Näsström T, Franquelim HG, Oliveira LM, Castanho MA, Lannfelt L, Bergström J, Ingelsson M, Quintas A, Sebastião AM, Lopes LV, Outeiro TF.
    J Neurosci. 2012 Aug 22;32(34):11750-62.
    PubMed

  • Antibodies against alpha-synuclein reduce oligomerization in living cells.
    Näsström T, Gonçalves S, Sahlin C, Nordström E, Screpanti Sundquist V, Lannfelt L, Bergström J, Outeiro TF, Ingelsson M.
    PLoS One. 2011;6(10):e27230. doi: 10.1371/journal.pone.0027230. Epub 2011 Oct 31.
    PubMed